Research Techniques & Certificates

R is a language primarily used for statistical computing and graphics, making it popular in data science.

Python is a versatile programming language used for web development, data analysis, AI, and more.

SAS is used for advanced analytics, business intelligence, and data management.

Java is a general-purpose programming language that is platform-independent and widely used in web applications.

Linux is an open-source operating system used for various computing environments, from servers to desktops.

Linear regression models the relationship between a dependent variable and one or more independent variables.

ANOVA (Analysis of Variance) is used to compare means across multiple groups to find if they significantly differ.

Mixed effect models analyze data that includes both fixed and random effects, useful for complex data structures.

Spatiotemporal data analysis studies data that changes over space and time, often used in environmental studies.

Longitudinal data analysis tracks data collected repeatedly over time, important for understanding changes within subjects.

Transcriptomics involves the study of RNA transcripts, allowing for insights into gene expression in various biological contexts.

Microbiome analysis through 16S rRNA sequencing is used to identify and compare bacteria within a sample, often for ecological and health-related studies.

Qiime2 is an open-source bioinformatics software for microbiome analysis, used to analyze and interpret high-throughput microbial community sequencing data.

3D Printing involves creating physical objects from digital models, often used in prototyping, manufacturing, and medical applications.

3D CAD (Computer-Aided Design) is used to create precise 3D models and designs, facilitating engineering, architecture, and manufacturing processes.

General cloning techniques are used to replicate DNA sequences and are fundamental for genetic manipulation and study.

Engineering plasmids involves constructing vector backbones for antigen delivery or genetic modification applications.

Inducible systems are designed to control gene expression, allowing targeted and timely gene activation or repression.

DNA mutagenesis techniques, such as allelic exchange and transduction mutagenesis, introduce targeted mutations to study gene function.

Gel electrophoresis, including RNA/DNA gels, SDS-PAGE, and 2D-PAGE, is used to separate biomolecules by size and charge.

DNA and RNA purification techniques are crucial for obtaining clean samples for downstream molecular analysis.

LPS profiling is the analysis of lipopolysaccharides (LPS) in bacterial cell membranes to understand their structure, composition, and role in immune response and pathogenicity.

qRT-PCR is a quantitative PCR technique used to measure RNA levels, allowing gene expression analysis.

Protein purification isolates specific proteins for functional and structural studies.

Western blotting is a technique used to detect specific proteins in a sample using antibodies.

ELISA is a plate-based assay used to detect and quantify substances such as proteins, antibodies, and hormones.

Bacteria cell fractionation and protein profiling involve separating bacterial cellular components to analyze and identify proteins, providing insights into their functions and roles within the cell.

Protein interaction assays investigate interactions between proteins, revealing signaling and structural pathways.

Beta-galactosidase assays measure enzyme activity, often used in gene expression studies.

General maintenance of bacterial phages includes processes like expansion, storage, and titration to ensure viability and usability.

Transduction is a method used to introduce genetic material into bacterial cells via bacteriophages, aiding in genetic studies and manipulation.

Standard bacterial cell culture techniques involve growing bacteria in controlled conditions to study their behavior, characteristics, and applications.

Techniques for isolating and identifying bacterial species are essential in microbiology for species characterization and study.

Biochemical tests are conducted to assess metabolic and chemical properties of bacteria, aiding in identification and classification.

The Rotating Wall Vessel (RWV) bioreactor, designed by NASA, provides a dynamic culture environment that simulates microgravity for advanced bacterial studies.

Standard monolayer cell culture techniques are used for cultivating cells such as intestinal epithelial cells, monocytes, and macrophages in a two-dimensional layer.

Three-dimensional (3D) organotypic intestinal cell cultures are developed using RWV bioreactors, providing a more physiologically relevant model for cell studies.

3D human intestinal epithelial co-cultures integrate human macrophages, creating a complex model to study host-microbe interactions.

Bacterial infection studies, such as the gentamicin protection assay, are used to investigate bacterial invasion and replication within host cells.

Tissue regeneration techniques explore the ability of cells and tissues to repair and renew, essential in regenerative medicine research.

General maintenance of *C. elegans* includes feeding, freezing, and recovery processes to ensure healthy and viable populations.

Egg preparation and larvae staging (L1 and L4) are essential for developmental studies and controlled experimental setups.

Infection studies in *C. elegans*, including TD50 determination, are used to analyze pathogen interactions and host response.

Light microscopy is a basic technique used to visualize cells and tissues with the aid of visible light.

Phase contrast microscopy enhances contrast in transparent specimens, making it ideal for observing live cells.

Epi-fluorescent microscopy uses fluorescence to illuminate specimens, allowing visualization of specific cellular components.

Laser Scanning Confocal Microscopy uses multiple laser channels, including options from 4 lasers to a white laser, to produce high-resolution, 3D images of specimens by selectively illuminating specific depths and capturing detailed optical sections.

Expertise in various chemical staining techniques for bacterial and mammalian cells, including immunohistochemistry and biofilm staining.

Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) are advanced techniques for high-resolution imaging of cellular structures.

Techniques for generating polyclonal antibodies using small laboratory animals such as rabbits, mice, and chickens.

Methods for administering substances orally, intraperitoneally, or intradermally to laboratory animals.

Techniques for studying animal infections and determining the lethal dose 50% (LD₅₀) in experimental animals.

Procedures for dissecting tissues in mice and chickens to study various biological processes and diseases.

Techniques for examining organs in mice and chickens to assess health, infection, and physiological conditions.

Techniques for isolating and quantifying microorganisms from animal tissues and organs, used for studying their presence and impact in various biological contexts.

Cardiac puncture is a technique used for obtaining blood samples from laboratory animals for analysis.

Standard biofilm culture techniques are used to grow single or mixed-species biofilms under controlled conditions.

Techniques for detecting extracellular polymeric substances (EPS) and other extracellular compounds in biofilms.

Live/dead staining techniques are used to assess the viability of cells within a biofilm, distinguishing live cells from dead ones.

Utilization of various spaceflight hardware to conduct biological experiments aboard the International Space Station (ISS).

Use of spaceflight-analogue cell culture systems to simulate microgravity and other space conditions for biological studies on Earth.

A spaceflight experiment aboard the ISS involves conducting scientific research in microgravity to study the effects on biological, physical, or technological systems.

Developing selective media involves creating a growth medium that supports the growth of specific microorganisms while inhibiting the growth of others.

Developing differential/selective media involves formulating a growth medium that not only supports the growth of specific microorganisms while inhibiting others but also allows for the differentiation between microbial types based on their biochemical properties.

Proficient in English, used for communication, academic writing, and professional collaboration.

Fluent in Korean, used for native communication, cultural understanding, and bilingual support.